Journal: Scientific Reports

Article Title: Fluorescence in situ hybridisation in Carnoy’s fixed tonsil tissue

doi: 10.1038/s41598-022-16309-w

Figure Lengend Snippet: Bacterial cell cultures used in this study to test and optimise FISH probes.

Article Snippet: Figure 3 Fluorescence in situ hybridisation staining of Streptococcus pyogenes ATCC 700,294, Pseudomonas aeruginosa ATCC BAA-47 and Escherichia coli K-12 DH5 alpha, ATCC PTA-4079 mixed cells from broth culture. ( A ) Nucleic material/DAPI in blue, ( B ) Eubacterial probe (EUB) in green, ( C ) Streptococcus spp . specific probe (STRC493) in red, ( D ) Pseudomonas spp . specific probe (PSE227) in yellow, ( E ) composite image of ( A – D ).

Techniques:

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Synergic antibacterial coatings combining titanium nanocolumns and tellurium nanorods.

doi: 10.1016/j.nano.2018.12.009

Figure Lengend Snippet: Fig. 6: E. coli colonies on the different samples after 16h. N=3. *p<0.005 versus control, **p<0.01 versus control.

Article Snippet: For the sake of comparison, the sputtered Ti thin films as well as commercial Ti disks from Goodfellow (thickness: 0.5 mm, code: 303-115-36) were also used: no significant differences between them were observed, so their results were averaged and jointly labeled D. Escherichia coli (strain K-12 HB101; Bio-Rad, Hercules, CA) and Staphylococcus aureus (subsp. aureus Rosenbach, ATCC® 12600TM; ATCC, Manassas, VA) bacteria were used.

Techniques: Control

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Antibodies investigated for cross-reactivity

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques:

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: List of antibodies and their modifications used to capture BSA, OVA, B. globigii spores, and E. coli O157:H7

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques:

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Standard curve of capture of B. globigii spores in PBST (A) and E. coli O157:H7 in meat extract and PBST (B) using 3-mm-diameter PEG-modified glass beads. Standard errors of the means are masked by the symbols. The y axis indicates absorbance.

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Modification

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Storage and blocking buffers used to test 3-mm-diameter anti- E. coli O157:H7 PEG-modified glass beads

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Blocking Assay

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Effect of blocking buffers on signal generation using 103 total E. coli O157:H7 cells over time stored at 25°C (⧫) and 7°C (◊). Standard errors of the means are masked by the symbols.

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Blocking Assay

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Confocal image of captured E. coli O157:H7 cells. Live cells are stained green, and dead cells are stained red. Slides were coated with PEG and anti-E. coli O157:H7 Ab.

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Staining

Journal: Applied Microbiology and Biotechnology

Article Title: An Extremely Sensitive Ultra-High Throughput Growth Selection Assay for the Identification of Amidase Activity

doi: 10.1007/s00253-024-13233-z

Figure Lengend Snippet: ( a ) The concept of the PABA growth selection assay for amidases. Acedoben, an amide-protected (red) derivative of p -aminobenzoic acid (PABA), can be deprotected by an amidase, resulting in the formation of PABA. Subsequently, PABA is available for folate synthesis, allowing PABA auxotrophs to grow. Sulfathiazole (ST), an antimetabolite of PABA, can be applied to fine-tune assay sensitivity. ( b ) Proof-of-concept for the PABA-growth selection assay. E. coli_∆pabA _pBAD_ aramd (aryl acylamidase, ArAmd) served as positive control for the hydrolysis of acedoben to PABA. The inactive ArAmd_S163A served as negative control. Cells of pre-cultures grown in LB medium were washed and resuspended in screening medium and used to inoculate 2 mL screening medium containing kanamycin and ampicillin at a 1:100,000 dilution. The cultures were incubated at 37 °C, 200 rpm for 48 h. Cells expressing ArAmd showed significant growth, whereas the inactive variant could only grow when 5 μM PABA (as the equivalent to the 5 μM acedoben used) was added

Article Snippet: The E. coli K12 auxotrophs ΔpabA (JW3323-KC; CGSC Strain #10483) and ΔpabB (JW1801-KC; CGSC Strain #9507) from the E. coli K-12 Keio Collection (Baba et al. ) were ordered from The National Bioresource Project E. coli Strain Office at the National Institute of Genetics in Mishima, Japan ( https://shigen.nig.ac.jp/ecoli/strain/ ).

Techniques: Selection, Positive Control, Negative Control, Incubation, Expressing, Variant Assay

Journal: Applied Microbiology and Biotechnology

Article Title: An Extremely Sensitive Ultra-High Throughput Growth Selection Assay for the Identification of Amidase Activity

doi: 10.1007/s00253-024-13233-z

Figure Lengend Snippet: Assay adaption to the agar plate format. Screening plates containing 250 nM ST were inoculated to evaluate the assay's performance across varying cell densities. The figure shows plates after incubation at 37 °C for 48 h with differing numbers of control cells: ( a ) ~ 600,000 negative control cells ( E. coli _ ∆pabA _pBAD_ aramd _S163A) and about 30 positive control cells ( E. coli _ ∆pabA _pBAD_ aramd) , ( b ) ~ 6 million negative control cells and 30 positive control cells, and ( c ) ~ 60 million negative control cells and 30 positive control cells. Despite the vast differences in background cell density, each plate consistently showed growth of only the positive control colonies, confirming the assay's specificity. ( d ) detail of the subsequent growth validation in liquid screening medium of three colonies (labeled 1–3) transferred from the plate shown in ( c ), a no-colony (NC) sample scraped from the plate without touching any colonies. Only the transferred colonies demonstrated growth, further verifying the accuracy of the assay in distinguishing positive controls under varying experimental conditions. The grown liquid cultures were verified to consist of E. coli_∆pabA _pBAD_ aramd and not E. coli _ ∆pabA _pBAD_ aramd _S163A by Sanger sequencing

Article Snippet: The E. coli K12 auxotrophs ΔpabA (JW3323-KC; CGSC Strain #10483) and ΔpabB (JW1801-KC; CGSC Strain #9507) from the E. coli K-12 Keio Collection (Baba et al. ) were ordered from The National Bioresource Project E. coli Strain Office at the National Institute of Genetics in Mishima, Japan ( https://shigen.nig.ac.jp/ecoli/strain/ ).

Techniques: Incubation, Control, Negative Control, Positive Control, Biomarker Discovery, Labeling, Sequencing